BBa_K934002BBa_K934002 Version 1 (Component)LuxR/3OC6HSL complex dependent LacI generator
BBa_S03736BBa_S03736 Version 1 (Component)pLac-lox-RBS-Tet (in pSB1A2)
BBa_K1654006BBa_K1654006 Version 1 (Component)LuxR (vibrio harveyi) induced expression of Alyteserin-1a
BBa_J10065BBa_J10065 Version 1 (Component)LuxI from V. fischeri C0061 with LVA tag removed
BBa_K145274BBa_K145274 Version 1 (Component)LuxI generator controlled by a AND-gate system.
BBa_K1718005BBa_K1718005 Version 1 (Component)LuxI and GFP behind a Bxb1 compatible genetic switch
BBa_K395102BBa_K395102 Version 1 (Component)GFP reporter repressed by LuxR and 3OC6HSL (K395005:K121013)
BBa_K395103BBa_K395103 Version 1 (Component)GFP reporter repressed by LuxR and 3OC6HSL (K395006:K121013)
BBa_K290001BBa_K290001 Version 1 (Component)constitutive RhlR with bicistronic LuxI - GFP controlled by pRhl
BBa_K132016BBa_K132016 Version 1 (Component)luxI+KanR-LVA+LacI+PL+KanR-LVA+aiiA+terminator
BBa_K584008BBa_K584008 Version 1 (Component)Lambda cI and LuxR regulated hybrid promotor + RBS + MelA + RBS + AFP + term
BBa_K180005BBa_K180005 Version 1 (Component)GoL - Primary plasmid (part 1)/RPS - Paper primary plasmid (part 1) [LuxR generator]
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.