BBa_K649001
BBa_K649001 Version 1 (Component)
GFP regulated by 3OC12-HSL and LasR

BBa_K566020
BBa_K566020 Version 1 (Component)
Mnt repressor regulated by pOmpC

BBa_K823041
BBa_K823041 Version 1 (Component)
PydfG promoter regulated by ecf41

BBa_K823042
BBa_K823042 Version 1 (Component)
PsspK promoter regulated by sigma G

BBa_K823048
BBa_K823048 Version 1 (Component)
PspoIVB promoter regulated by sigma G

BBa_J100099
BBa_J100099 Version 1 (Component)
A promoter (CydAB) activated by the FNR enzyme

BBa_K748004
BBa_K748004 Version 1 (Component)
P3+RBS+GFP+T. P3 can be activated by phosphorylated AgrA.

BBa_K748005
BBa_K748005 Version 1 (Component)
P2+RBS+GFP+T. P2 can be activated by phosphorylated AgrA.

BBa_K748008
BBa_K748008 Version 1 (Component)
P2 promoter. P2 promoter can be activated by phosphorylated AgrA.

BBa_K748009
BBa_K748009 Version 1 (Component)
P3 promoter. P3 can be activated by phosphorylated AgrA.

BBa_K887011
BBa_K887011 Version 1 (Component)
DNA program : specific DNA sequence which can be recognized by zinc fingers

BBa_K750000
BBa_K750000 Version 1 (Component)
pBADGLT:unstable gfp expression device activated by arabinose

BBa_K750001
BBa_K750001 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 1.0 strength)

BBa_K750002
BBa_K750002 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.6 strength)

BBa_K750003
BBa_K750003 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.3 strength)

BBa_K750004
BBa_K750004 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.07 strength)

TBY
BBa_K897720 Version 1 (Component)
Antisense FtsZ protected by paired termini structure under T7 promoter

BBa_K750005
BBa_K750005 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.01 strength)

BBa_K750006
BBa_K750006 Version 1 (Component)
LuxR expression device activated by arabinose

BBa_K737023
BBa_K737023 Version 1 (Component)
Promoter that can be induced by phosphate.

BBa_K737026
BBa_K737026 Version 1 (Component)
Promoter that can be induced by phosphate and carbon starvation.

BBa_K737035
BBa_K737035 Version 1 (Component)
Spot42 generator controlled by IPTG

BBa_K737036
BBa_K737036 Version 1 (Component)
Spot42 generator controlled by aTc

BBa_K737038
BBa_K737038 Version 1 (Component)
galK::GFP generator ligated to sRNA device controlled by IPTG

BBa_K737039
BBa_K737039 Version 1 (Component)
galK::GFP generator ligated to sRNA device controlled by aTc

BBa_K818100
BBa_K818100 Version 1 (Component)
Promoter sboA, upregulated by rotten meat volatiles in B. subtilis

BBa_K818200
BBa_K818200 Version 1 (Component)
Regulator fnr, upregulated by rotten meat volatiles in B. subtilis

P-alsT
BBa_K818300 Version 1 (Component)
Promoter alsT, repressed by TnrA during conversion of NH4 to Glutamine in B. subtilis.

BBa_K759001
BBa_K759001 Version 1 (Component)
Aggregation Module inducible by arabinose in E.coli.

BBa_K908015
BBa_K908015 Version 1 (Component)
Microcin B17, Gene A flanked by rbs and Attc sequence

BBa_K908016
BBa_K908016 Version 1 (Component)
Microcin B17, Gene B flanked by Attc sequence and rbs

BBa_K908017
BBa_K908017 Version 1 (Component)
Microcin B17, Gene C flanked by Attc sequence and rbs

BBa_K908018
BBa_K908018 Version 1 (Component)
Microcin B17, Gene F flanked by Attc sequence and rbs

BBa_K908019
BBa_K908019 Version 1 (Component)
Microcin C7, Gene E flanked by Attc sequence and rbs

BBa_K908020
BBa_K908020 Version 1 (Component)
Microcin C7, Gene C flanked by Attc sequence and rbs

BBa_K908021
BBa_K908021 Version 1 (Component)
Microcin C7, Gene F flanked by Attc sequence and rbs

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K098986
BBa_K098986 Version 1 (Component)
lac QPI driven by high promoter

BBa_K098985
BBa_K098985 Version 1 (Component)
lac QPI driven by low promoter

BBa_K098981
BBa_K098981 Version 1 (Component)
tet QPI driven by high promoter

BBa_K098980
BBa_K098980 Version 1 (Component)
tet QPI driven by low promoter

BBa_K086001
BBa_K086001 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

BBa_K086002
BBa_K086002 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP

BBa_K086003
BBa_K086003 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

BBa_K116500
BBa_K116500 Version 1 (Component)
OmpF promoter that is activated or repressesed by OmpR according to osmolarity.

BBa_K116001
BBa_K116001 Version 1 (Component)
nhaA promoter, that can be regulated by pH and nhaR protein.

BBa_J04430
BBa_J04430 Version 1 (Component)
GFP coding device switched on by IPTG

pLac+GFP
BBa_I763004 Version 1 (Component)
GFP coding device switched on by IPTG

BBa_K142048
BBa_K142048 Version 1 (Component)
promotorless lacI expression cassette followed by constitutively active TetR generator