Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wenjie Wu and Peiran Zhang
Date created: 2012-09-18 11:00:00
Date modified: 2015-05-08 01:13:07
galK::GFP generator ligated to sRNA device controlled by IPTG
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K737038_sequence (Version 1) |
Description
Small RNA Spot42 controls the GFP mRNA translation by blocking the RBS in galK.The galK and E0040 is fused together using ClaI site to avoid stop condon in standard scar.J23106 is a moderate promotor,and galK???s RBS is a moderate one.[J23106,P0412]is a LacI generator, which represses lac promotor and can be induced by IPTG.
IPTG concentration gradient results in different sRNA Spot42 levels in cells, which can be monitored by our galK::GFP generator,where galK is the RBS of GFP and the target for Spot42.
Notes
References:[1]Johannes H. Urban and Jo?? rg Vogel, Translational control and target recognition by Escherichia coli small RNAs in vivo, Nucleic Acids Research, 2007, Vol. 35, No. 3
Source
No| Access | Instance | Definition |
| public public public public public public public | BBa_J23106 BBa_P0412 BBa_R0011 BBa_K737058 BBa_J23106 BBa_K737033 BBa_K737066 | BBa_J23106 BBa_P0412 lacI+pL BBa_K737058 BBa_J23106 BBa_K737033 BBa_K737066 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23106 BBa_P0412 BBa_R0011 BBa_K737058 BBa_J23106 BBa_K737033 BBa_K737066 | 1,35 44,1351 1360,1413 1423,1531 1540,1574 1583,1709 1718,2434 | BBa_J23106 BBa_P0412 lacI+pL BBa_K737058 BBa_J23106 BBa_K737033 BBa_K737066 |