BBa_K809108
BBa_K809108 Version 1 (Component)
Efficiency test of Q0255 Terminator

BBa_I731014
BBa_I731014 Version 1 (Component)
The luxR based receiver, F2620 (formerly I13270), controls the production of mCherry

BBa_K415005
BBa_K415005 Version 1 (Component)
pLux/cI-OR : RBS-mCherry : Term : p(tetR) : RBS-luxR : Term

BBa_I6038
BBa_I6038 Version 1 (Component)
Promoter O_H Test R0052.E0430

BBa_K1606010
BBa_K1606010 Version 1 (Component)
Kumamax enzyme that gluten updated m-cherry reporter device

BBa_I0416
BBa_I0416 Version 1 (Component)
RBS Test R0011.B0033.E0030.B0015

BBa_I0418
BBa_I0418 Version 1 (Component)
RBS Test R0011.B0030.E0030.B0015

BBa_I0419
BBa_I0419 Version 1 (Component)
RBS Test R0011.B0032.E0030.B0015

BBa_I0417
BBa_I0417 Version 1 (Component)
RBS Test R0011.B0034.E0030.B0015

BBa_I0415
BBa_I0415 Version 1 (Component)
RBS Test R0011.B0031.E0030.B0015

BBa_I0413
BBa_I0413 Version 1 (Component)
RBS Test R0040.B0030.E0030.B0015

BBa_I0411
BBa_I0411 Version 1 (Component)
RBS Test R0040.B0033.E0030.B0015

BBa_I0410
BBa_I0410 Version 1 (Component)
RBS Test R0040.B0031.E0030.B0015

BBa_I0412
BBa_I0412 Version 1 (Component)
RBS Test R0040.B0034.E0030.B0015

BBa_I9107
BBa_I9107 Version 1 (Component)
test (tp901-cI nor_output) R0075.E0432

BBa_I12029
BBa_I12029 Version 1 (Component)
Test of pBAD/araC promoter (I0500)

BBa_I12026
BBa_I12026 Version 1 (Component)
Test of BBa_R0011 (LacI regulated) using YFP

BBa_K415011
BBa_K415011 Version 1 (Component)
PtetR : RBS : LuxR : Term : PluxR/cI-OR : RBS : mCherry : Term : Plux/cI-OR : RBS : LuxI

BBa_I13901
BBa_I13901 Version 1 (Component)
Synchronized Brickilator Test Construct (I13201.Q04400.I0461)

BBa_J69122
BBa_J69122 Version 1 (Component)
C12 AHL Receiver test construct with EYFP Reporter

BBa_K165100
BBa_K165100 Version 1 (Component)
Gli1 bs + LexA bs + mCYC + LexA repressor (mCherryx2 tagged) on pRS304*

[OriTR]+[R
BBa_K1439002 Version 1 (Component)
This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid mobility.

BBa_K165101
BBa_K165101 Version 1 (Component)
Zif268-HIV bs + LexA bs + mCYC + Zif268-HIV repressor (mCherryx2 tagged) on pRS304*

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.