SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K156009
BBa_K156009 Version 1 (Component)
OFP (orange fluorescent protein)

SBFP2
BBa_K156010 Version 1 (Component)
SBFP2 (strongly enhanced blue fluorescent protein)

Bioplastic
BBa_K156013 Version 1 (Component)
phaB1 (acetyacetyl-CoA reductase)

BBa_K116201
BBa_K116201 Version 1 (Component)
ureD promoter from <I>P mirabilis<I>

BBa_M11006
BBa_M11006 Version 1 (Component)
ArsC arsenate reductase protein

BBa_M11010
BBa_M11010 Version 1 (Component)
Thioredoxin Peroxidase II, Cricetulus griseus, mRNA Coding

BBa_M11079
BBa_M11079 Version 1 (Component)
Copper binding paired with Polystyrene binding

BBa_J70315
BBa_J70315 Version 1 (Component)
Test red bs part

BBa_J70428
BBa_J70428 Version 1 (Component)
{7,12:15,10} red version

BBa_J70498
BBa_J70498 Version 1 (Component)
Red {-1,4;0,5} PsrI part (for non protein/AG parts)

BBa_K177042
BBa_K177042 Version 1 (Component)
Yellow Fluorescent Protein under RBS.3 (medium)

BBa_K233310
BBa_K233310 Version 1 (Component)
Promoter-RBS-GFP- constitutive green fluorescent protein device

BBa_K216006
BBa_K216006 Version 1 (Component)
pentaerythritol tetranitrate reductase with native rbs

BBa_K216012
BBa_K216012 Version 1 (Component)
PompC+EYFP+PETN reductase

BBa_K248002
BBa_K248002 Version 1 (Component)
nirK (nitrite reductase)

IFP
BBa_K256008 Version 1 (Component)
Infrared Protein (IFP 1.4)

BBa_K247000
BBa_K247000 Version 1 (Component)
monomeric RFP: Red Fluorescent Protein.

BBa_K203125
BBa_K203125 Version 1 (Component)
SREBP regulated promoter, predicted, tested HB8

BBa_K203126
BBa_K203126 Version 1 (Component)
SREBP regulated promoter, predicted, tested HB9

BBa_K256033
BBa_K256033 Version 1 (Component)
Infrared signal reporter (J23119:IFP:J23119:HO1)

BBa_K187299
BBa_K187299 Version 1 (Component)
mreD ORF, Forward Primer

BBa_K187300
BBa_K187300 Version 1 (Component)
mreD ORF Reverse Primer