Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Forrest Purser
Date created: 2009-04-17 11:00:00
Date modified: 2015-05-08 01:13:53
Copper binding paired with Polystyrene binding
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_M11079_sequence (Version 1) |
Description
Contains circularly permuted OmpX protein that enables E.coli to bind to a polystyrene surface. This sequence also contains the surface display protein OmpC, which has been modified to include a copper binding protein (CutA) on the extracellular loop of the membrane protein. This system is designed to be used for removing copper from solution by adding E. coli bound to a polystyrene surface and containing the copper binding mechanism to the solution.Notes
The OmpC BioBrick would need to be mutated to remove EcoRI and a SpeI sites. There were no other special design considerations.Source
BioBricks found in the Registry of Standard Biological Parts were used to make this composite sequence.| Access | Instance | Definition |
| public public public public public public public public public public | BBa_R0040 BBa_I11010 BBa_I728500 BBa_B0010 BBa_B0012 BBa_R0040 BBa_I11010 BBa_M11078 BBa_B0010 BBa_B0012 | p(tetR) BBa_I11010 CPX BBa_B0010 BBa_B0012 p(tetR) BBa_I11010 BBa_M11078 BBa_B0010 BBa_B0012 |