BBa_K549039BBa_K549039 Version 1 (Component)fur is an iron-dependent regulator from N. meningitidis
BBa_K549005BBa_K549005 Version 1 (Component)LacI promotor fused with the iron dependent regulator fur
BBa_K526002BBa_K526002 Version 1 (Component)Temperature dependent Ribo-Switch fused to GFP
BBa_K934002BBa_K934002 Version 1 (Component)LuxR/3OC6HSL complex dependent LacI generator
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
BBa_M11007BBa_M11007 Version 1 (Component)NADH-dependent butanol dehydrogenase A (bdhA/BDH I)
BBa_M11008BBa_M11008 Version 1 (Component)NADH-dependent butanol dehydrogenase B (bdhB/BDH II)
BBa_K289004BBa_K289004 Version 1 (Component)ADH(Alcohol dehydrogenase)
BBa_K345000BBa_K345000 Version 1 (Component)Taq DNA-dependent polymerase
BBa_K334000BBa_K334000 Version 1 (Component)Oxygen Dependent Degradation Domain