T25 domain
BBa_K1088056 Version 1 (Component)
T25 domain of CyaA from Bordetella pertussis

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_M31786
BBa_M31786 Version 1 (Component)
1st half of Gene III- preBamHI cut

BBa_K648103
BBa_K648103 Version 1 (Component)
RecA with mutation of Arg 243

CsgD
BBa_K1019001 Version 1 (Component)
CsgD: positive regulator of curlin genes

BBa_K648102
BBa_K648102 Version 1 (Component)
RecA with mutation of Lys 286

BBa_K1114003
BBa_K1114003 Version 1 (Component)
The MoClo format of BBa_J23103 with AB fusion sites.

PrtDEF
BBa_K258007 Version 1 (Component)
Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi

BBa_K831011
BBa_K831011 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

BBa_K831012
BBa_K831012 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

BBa_I733005
BBa_I733005 Version 1 (Component)
Produce GFP in presence of AHL

BBa_K1725317
BBa_K1725317 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_I12026
BBa_I12026 Version 1 (Component)
Test of BBa_R0011 (LacI regulated) using YFP

BBa_J11009
BBa_J11009 Version 1 (Component)
variant of J11005 (low temp color) with stronger RBS

BBa_K1947017
BBa_K1947017 Version 1 (Component)
We verify the expression effect of Mms13.

BBa_J14462
BBa_J14462 Version 1 (Component)
Composite part comprised of J13002 and J04650

BBa_K1051262
BBa_K1051262 Version 1 (Component)
The measurement pathway of degradation tag K1051208.

BBa_K1051260
BBa_K1051260 Version 1 (Component)
The measurement pathway of degradation tag K1051206.

BBa_K1051261
BBa_K1051261 Version 1 (Component)
The measurement pathway of degradation tag K1051207.

BBa_J14459
BBa_J14459 Version 1 (Component)
Composite part comprised of J04500 and J04630

BBa_J14464
BBa_J14464 Version 1 (Component)
Composite part comprised of J13002 and J04630

BBa_K1676088
BBa_K1676088 Version 1 (Component)
Mutant 12 of LDH and wildtype LDP

BBa_K783048
BBa_K783048 Version 1 (Component)
This is a MoClo converted version of BBa_B0031

BBa_K783039
BBa_K783039 Version 1 (Component)
This is a MoClo converted version of BBa_J23101

BBa_K783049
BBa_K783049 Version 1 (Component)
This is a MoClo converted version of BBa_B0032

BBa_K783040
BBa_K783040 Version 1 (Component)
This is a MoClo converted version of BBa_J23110

BBa_K1413045
BBa_K1413045 Version 1 (Component)
A fusion of Universal Transposon Plasmid and pSB1C3

BBa_K783034
BBa_K783034 Version 1 (Component)
This is a MoClo converted version of BBa_J23114

BBa_K351006
BBa_K351006 Version 1 (Component)
first Ig-like region of Human basic FGFR

BBa_K783050
BBa_K783050 Version 1 (Component)
This is a MoClo converted version of BBa_B0033

BBa_K783038
BBa_K783038 Version 1 (Component)
This is a MoClo converted version of BBa_J23100

BBa_K1676091
BBa_K1676091 Version 1 (Component)
Mutant 15 of LDH and wildtype LDP

BBa_K1676086
BBa_K1676086 Version 1 (Component)
Mutant 10 of LDH and wildtype LDP

BBa_K1676080
BBa_K1676080 Version 1 (Component)
Mutant 4 of LDH and wildtype LDP

BBa_K581003
BBa_K581003 Version 1 (Component)
SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)

BBa_M31116
BBa_M31116 Version 1 (Component)
M13 Gene VIII terminator and promoter of Gene X

BBa_K077041
BBa_K077041 Version 1 (Component)
AiiA and cII under control of plac promotor

KanR-BA-Bf
BBa_K349012 Version 1 (Component)
KanR-BA-BfuAI (for construction of BA BioBytes 2.0)

BBa_K648123
BBa_K648123 Version 1 (Component)
RecA with mutation of Lys 286 and Arg 243

BBa_K1088057
BBa_K1088057 Version 1 (Component)
T25 domain of bacterial two-hybrid system (IPTG inducible)

BBa_K1172914
BBa_K1172914 Version 1 (Component)
Part 2 of the Biosafety-System TetOR alive (TetO GFP)

AraC_TEV-F
BBa_K627008 Version 1 (Component)
Fusion part of arabinose-inducible induction system and the TEV protease

BBa_I758601
BBa_I758601 Version 1 (Component)
Screen for binding affinity of mutant cI lambda to promotor sites

BBa_I758600
BBa_I758600 Version 1 (Component)
Screen for binding affinity of mutant cI lambda to promotor sites

BBa_K1351019
BBa_K1351019 Version 1 (Component)
Reverse complementary RNA sequence which binds the mRNA of the SdpI immunity

ssTorA_CS-
BBa_K627012 Version 1 (Component)
Fusion of TorA sig-seq, TEV protease cleavage site and b-lactamase

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K1113411
BBa_K1113411 Version 1 (Component)
Targeting sequence for the delivery of the LacZ gene to the Carboxysome