BBa_K777103
BBa_K777103 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23105

BBa_K777104
BBa_K777104 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23106

BBa_K777105
BBa_K777105 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23109

BBa_K777106
BBa_K777106 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23112

BBa_K777107
BBa_K777107 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23113

BBa_K777108
BBa_K777108 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23114

BBa_K769002
BBa_K769002 Version 1 (Component)
Kinase domain of EnvZ

CBDcex(T7)
BBa_K863102 Version 1 (Component)
Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K098984
BBa_K098984 Version 1 (Component)
lac inducible mtrB (high expression of QPI)

BBa_K098983
BBa_K098983 Version 1 (Component)
lac inducible mtrB (low expression of QPI)

BBa_K157002
BBa_K157002 Version 1 (Component)
Transmembrane region of the EGF-Receptor (ErbB-1)

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K125810
BBa_K125810 Version 1 (Component)
slr2016 signal sequence + GFP fusion for secretion of GFP

OriTR
BBa_J01003 Version 1 (Component)
OriT-R (Origin of transfer for the R-plasmid nic region)

BBa_K156009
BBa_K156009 Version 1 (Component)
OFP (orange fluorescent protein)

BBa_K156024
BBa_K156024 Version 1 (Component)
RBS + OFP

BBa_K116510
BBa_K116510 Version 1 (Component)
GFP under control of pOmpf promoter(K116500+E0240)

BBa_K142024
BBa_K142024 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197A) and TetR expression cassette

BBa_K142025
BBa_K142025 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197F) and TetR expression cassette

BBa_K142026
BBa_K142026 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (T276A) and TetR expression cassette

BBa_K142027
BBa_K142027 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (T276F) and TetR expression cassette

BBa_K142028
BBa_K142028 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197A, T276A)/TetR expression cassette

BBa_K142029
BBa_K142029 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197A, T276F)/TetR expression cassette

BBa_K142030
BBa_K142030 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197F, T276A)/TetR expression cassette

BBa_K142031
BBa_K142031 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197F, T276F)/TetR expression cassette

BBa_K116501
BBa_K116501 Version 1 (Component)
RpaA(regulator of phycobilisome-associated)

BBa_K116503
BBa_K116503 Version 1 (Component)
GFP under control of pOmpC promoter.R0082+E0240

BBa_K116520
BBa_K116520 Version 1 (Component)
GFP under control of RpaA activated pOmpF promoter

BBa_K116523
BBa_K116523 Version 1 (Component)
GFP under control of RpaA activated pOmpC promoter

BBa_K116000
BBa_K116000 Version 1 (Component)
A homolog of OCT3 (organic cation transporter 3) from E coli. K12

BBa_K116002
BBa_K116002 Version 1 (Component)
measurement of NhaA promoter activity

BBa_K089005
BBa_K089005 Version 1 (Component)
-35 to Tc start site of phaC

BBa_K089006
BBa_K089006 Version 1 (Component)
-663 to Tc start site of phaC

BBa_K156035
BBa_K156035 Version 1 (Component)
Promoter + RBS + OFP

BBa_K112125
BBa_K112125 Version 1 (Component)
OriV origin of replication

BBa_K152004
BBa_K152004 Version 1 (Component)
Promoterless Synthetic Operon of CrtE,B,I,Y

BBa_K152005
BBa_K152005 Version 1 (Component)
Promoterless Synthetic Operon of CrtE,B,I,Y and GFP

BBa_K152007
BBa_K152007 Version 1 (Component)
Synthetic operon of CrtEBIY and FrdBCD and GFP

BBa_K091209
BBa_K091209 Version 1 (Component)
lacIQ promoter under the control of the represseor LacI

BBa_K091210
BBa_K091210 Version 1 (Component)
lacIQ1 promoter under the control of LacI repressor

BBa_K091211
BBa_K091211 Version 1 (Component)
lacIQ promoter under the control of LacI_I12 repressor

BBa_K091212
BBa_K091212 Version 1 (Component)
lacI promoter under the control of wild-type Lac repressor

BBa_J70031
BBa_J70031 Version 1 (Component)
Linker for BioScaffold Parts, example of Gamma BioScaffold part

BBa_K091213
BBa_K091213 Version 1 (Component)
LacI X86 and pLac promoter upstream of GFP

BBa_J70121
BBa_J70121 Version 1 (Component)
R0040 regulated expression of a lacZa.GFP fusion protein

BBa_M11082
BBa_M11082 Version 1 (Component)
ZntR:Zn(II)-responsive regulator of zntA (also MerR transcriptional regulator)

BBa_M11051
BBa_M11051 Version 1 (Component)
Captures light energy and uses it to move protons across the membrane out of the cell.

BBa_M11085
BBa_M11085 Version 1 (Component)
E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o