Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Johnston Monje
Date created: 2008-10-30 12:00:00
Date modified: 2015-05-08 01:10:49
Promoterless Synthetic Operon of CrtE,B,I,Y and GFP
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K152005_sequence (Version 1) |
Description
This operon provides the full set of bacterial open reading frames necessary for beta carotene production with a GFP and termination stop signals tacked on at the end for good measure! crtE,B,I,Y coding regions from Erwinia uredovora each with a rbs preceeding the open reading frame. Likewise, the RBS-GFP-Term from biobrick BBa_E0240 is included on the end to provide a visual signal affirming transcription of preceeding 4 genes. There is no promoter on this construct, and after this GFP biobrick, no more genes can be added.Notes
The synthetic operon needed a transcriptional termination signal, as well as GFP to visualize complete transcript production, so BBa_E0240 was used. No more genes can be added to this operon with this GFP on the end.Source
This operon is made from biobricks BBa_K152001 - BBa_K152003, the biobrick BBa_K118005 which was sent to us by iGEM Edinburg, and BBa_E0240.| Access | Instance | Definition |
| public public public public public public public public public | BBa_K152001 BBa_K152002 BBa_J15001 BBa_K118003 BBa_K152003 BBa_B0032 BBa_E0040 BBa_B0010 BBa_B0012 | BBa_K152001 BBa_K152002 BBa_J15001 BBa_K118003 BBa_K152003 BBa_B0032 GFP BBa_B0010 BBa_B0012 |