Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Johnston Monje
Date created: 2008-10-30 12:00:00
Date modified: 2015-05-08 01:10:49
Promoterless Synthetic Operon of CrtE,B,I,Y
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K152004_sequence (Version 1) |
Description
This operon provides the full set of bacterial open reading frames necessary for beta carotene production. crtE,B,I,Y coding regions from Erwinia uredovora each with a rbs preceeding the open reading frame. This synthetic operon could be made by ligating together the starter biobrick containing CrtE cut with SpeI and PstI, then ligating in the next XbaI/PstI cut fragment. There is no promoter or trancription termination signal on this construct.Notes
To save time, the cloning was done in parrallel. CrtE was ligated to B, and crtI was ligated to crtY. CrtIY was then cut and ligated to CrtEB to yield CrtEBIY.Source
This operon is made from biobricks BBa_K152001 - BBa_K152003 and the biobrick BBa_K118005 which was sent to us by iGEM Edinburg.| Access | Instance | Definition |
| public public public public public | BBa_K152001 BBa_K152002 BBa_J15001 BBa_K118003 BBa_K152003 | BBa_K152001 BBa_K152002 BBa_J15001 BBa_K118003 BBa_K152003 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K152001 BBa_K152002 BBa_J15001 BBa_K118003 BBa_K152003 | 1,928 937,1846 1855,1864 1871,3352 3361,4528 | BBa_K152001 BBa_K152002 BBa_J15001 BBa_K118003 BBa_K152003 |