Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Johnston Monje
Date created: 2008-10-30 12:00:00
Date modified: 2015-05-08 01:10:49
Synthetic operon of CrtEBIY and FrdBCD and GFP
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K152007_sequence (Version 1) |
Description
A limiting factor behind carotenoid accumulation in many organisms is sink strength. Carotenoids are lipophilic, so it is hoped that increased membrane might provide more storage capacity for beta carotene accumulation. In addition to the four Crt genes required for beta carotene production, three of the four genes in the FrdABCD operon were included to increase membrane production, while GFP was added to visualize transcript production and ensure transcription termination.Notes
This operon contains 8 genes and was harder to put together than smaller constructs. Nothing specific though.Source
Composite of other biobricks| Access | Instance | Definition |
| public public public public public public | BBa_K152001 BBa_K152002 BBa_K118005 BBa_K152006 BBa_K152003 BBa_E0240 | BBa_K152001 BBa_K152002 BBa_K118005 BBa_K152006 BBa_K152003 GFP report |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K152001 BBa_K152002 BBa_K118005 BBa_K152006 BBa_K152003 BBa_E0240 | 1,928 937,1846 1855,3352 3361,4905 4914,6081 6090,6965 | BBa_K152001 BBa_K152002 BBa_K118005 BBa_K152006 BBa_K152003 GFP report |