Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hans
Date created: 2004-07-21 11:00:00
Date modified: 2015-08-31 04:07:31
Test Construct of BBa_I12006 promoter
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_I12020_sequence (Version 1) |
Description
This construct will allow the tester to switch YFP on and off if the BBa_I12006 promoter is working. The system will fluoresce when there is no p22 cII present in the system and when ATC is added. However, upon addition of IPTG, the system should cease fluorescing.Notes
The test construct was built using BioBrick parts that were available and working. The premise behind this test construct is that the absence of p22 cII will yield a constant promotion of TetR, which will inhibit production of lambda cI. However, upon addition of ATC, the ATC binds to the TetR, thus allowing lambda cI to be promoted. The lambda cI will then bind to the OR-1 and OR-2 regions of BBa_I12006 and thus, promoting the production of Yellow Flourescence Protein.Similarly, the absence of p22 cII will promote the production of LacI in a different subsystem. The LacI will normally inhibit the 434 cI repressor. However, once IPTG is added, the IPTG will bind to the LacI, hence freeing the promoters of the QPI (Quad Part Inverter) and promoting production of 434 cI repressor. The 434 cI repressor will then bind to the O-R3 region of BBa_I12006, hence, inhibiting production of Yellow Flourescence Protein, and thus, turning the switch off.
| Access | Instance | Definition |
| public public public public | BBa_I12022 BBa_I12023 BBa_I12006 BBa_E0430 | BBa_I12022 BBa_I12023 Prm + BBa_E0430 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I12022 BBa_I12023 BBa_I12006 BBa_E0430 | 1,2290 2299,4200 4209,4290 4299,5176 | BBa_I12022 BBa_I12023 Prm + BBa_E0430 |