BBa_I52001

BBa_I52001 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_I52001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Reshma Shetty

Date created: 2007-01-29 12:00:00

Date modified: 2015-08-31 04:07:42

ccdB and nonfunctional pUC19 derived high copy origin

Details

• Types: DnaRegion
• Roles: Plasmid; plasmid
• Sequences: BBa_I52001_sequence (Version 1)

Description

This part is designed to be located in the multiple cloning site of low copy BioBrick vectors. BBa_P1016 provides selection against non-insert containing clones and BBa_I50020 enables the base vector to be propagated at high copy for ease of purification

Notes

This part is a composite part of BBa_P1016 and BBa_I50020. It is designed to be placed in the multiple cloning site of BioBricks vectors. Vectors with this part in the multiple cloning site will be selected against during BioBricks assembly because BBa_P1016 is lethal to most cell strains. Also, BBa_I50020 enables the vector to be propagated at high copy regardless of the other origins on the vector.

Source

ccdB and pUC19

Components

Access	Instance	Definition
public	BBa_P1016	ccdB
public	BBa_I50020	pUC19 ori

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_P1016	1,416	ccdB
BBa_I50020	417,1090	pUC19 ori

Other Properties

• igem#experience: Issues
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I52001/1

Member of these Collections

• //plasmidbackbone/component/defaultinsert
• iGEM Parts Registry