Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dave Curran
Date created: 2007-10-22 11:00:00
Date modified: 2015-08-31 04:08:01
With AHL, light -> area of no GFP.
| Types | DnaRegion |
| Roles | Project engineered_region |
| Sequences | BBa_I737012_sequence (Version 1) |
Description
There was some amniguity on the registry site about which parts were OmpC, and which were OmpF. The two have opposing functionality, so this was an important distinction. Looking at the literature, there was also some question as to which promoter we would be able to use in our system, though we wanted to use OmpF. This is the reversal of the logic circuit of the 2007 University of Calgary project built with OmpC, and so requires the light sensor (M30109) to function. After addition of AHL to the media, the cells will be responsive to 650 nm light, and begin making GFP. When they sense light, they will stop expressing GFP (E0040), and will remain inactive until any AHL in the media breaks down. In the absence of either AHL, the bacteria should do nothing.Notes
The protein LuxR is present in the system twice; it should be expressed first upon exposure to light, and should then activate expression of yet more LuxR. This allows continued expression of GFP even when the light is removed.Source
This part was made using existing biobrick parts.| Access | Instance | Definition |
| public public public public | BBa_R0083 BBa_A340620 BBa_S01414 BBa_I13504 | OmpR BBa_A340620 BBa_S01414 BBa_I13504 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0083 BBa_A340620 BBa_S01414 BBa_I13504 | 1,78 87,1085 1094,1892 1901,2775 | OmpR BBa_A340620 BBa_S01414 BBa_I13504 |