Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vladislav Lavrovsky
Date created: 2007-10-25 11:00:00
Date modified: 2015-08-31 04:08:01
Constitutively expressed Agarase part.
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_I737021_sequence (Version 1) |
Description
This part constituatively expresses agarase. It is used to produce e. coli which degrade the agar substrate that they are normally grown on. Typical rate of degradation is low, it takes two to three days to degrade 4mm of agar underneath the colonies.Notes
There are EcorI and PstI sites. This limits construction. Future versions will be mutated by site-directed mutagenesis. The flanks of the gene were included for the signal peptide. Agarase is exported in the source organism using the TAT export system with e. coli K12 has, unfortunately so far e. coli dont degrade agar when transformed with this part. We are not sure whether it is a problem with expression or export.Source
Cloned from Pseudoalteromonas atlantica T6C.| Access | Instance | Definition |
| public public public | BBa_R0040 BBa_B0034 BBa_I737020 | p(tetR) BBa_B0034 BBa_I737020 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0040 BBa_B0034 BBa_I737020 | 1,54 63,74 83,2673 | p(tetR) BBa_B0034 BBa_I737020 |