BBa_I746914

BBa_I746914 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I746914

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Stefan Milde

Date created: 2008-09-30 11:00:00

Date modified: 2015-08-31 04:08:05

pbad promoter driving 6-his tagged superfolder GFP

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_I746914_sequence (Version 1)

Description

This part is used for purification of superfolder GFP (for source and other information about this new GFP variant see its part description: I746916) via its 6-his tag. It is driven by the arabinose - sensitive pbad promoter. Induction was with 1mM arabinose for overnight growth of a 50ml culture for small scale purification the next day.

Notes

no special considerations.

Source

The part was created from intermediates by standard assembly.

Components

Access	Instance	Definition
public	BBa_I0500	pBad/araC
public	BBa_B0034	BBa_B0034
public	BBa_I746919	BBa_I746919
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I0500	1,1210	pBad/araC
BBa_B0034	1219,1230	BBa_B0034
BBa_I746919	1237,1974	BBa_I746919
BBa_B0010	1983,2062	BBa_B0010
BBa_B0012	2071,2111	BBa_B0012

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I746914/1

Member of these Collections

• iGEM Parts Registry