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ZF test

BBa_J05000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J05000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: NOR-Group

Date created: 2005-08-28 11:00:00

Date modified: 2015-08-31 04:08:15

Zinc Finger Protein Tester

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Details

Types	DnaRegion
Roles	engineered_region Measurement
Sequences	BBa_J05000_sequence (Version 1)

Description

Tester to investigate zinc finger proteins (ZFP) as a negative regulator of expression. Lac promoter controls expression of a ZFP and a reporter (i.e. RFP) to confirm expression. Lambda promoter constitutively expresses GFP unless ZFP binds to the binding site after the lambda promoter.

Notes

Promotor RBS Zinc_Finger RBS RFP Terminator Promotor ZF-Binding_site1 ZF-Binding_site2 RBS GFP Terminator

Components

Access	Instance	Definition
public public public public public public public	BBa_R0010 BBa_B0034 BBa_J05100 BBa_I13507 BBa_R0051 BBa_J05211 BBa_I13504	LacI BBa_B0034 BBa_J05100 BBa_I13507 cI lam BBa_J05211 BBa_I13504

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010 BBa_B0034 BBa_J05100 BBa_I13507 BBa_R0051 BBa_J05211 BBa_I13504	1,200 209,220 227,535 544,1404 1413,1461 1470,1493 1502,2376	LacI BBa_B0034 BBa_J05100 BBa_I13507 cI lam BBa_J05211 BBa_I13504