Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ETH Zurich iGEM Team 2005
Date created: 2005-09-20 11:00:00
Date modified: 2015-08-31 04:08:15
Sensing Device A (cI)
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_J05500_sequence (Version 1) |
Description
The input system for the iGEM2005 ETH "Counter" project: its purpose is to regulate cI production dependent on an external event. It works somehow like an inverter:STATE 1: when IPTG is absent, the constitutively produced lacI protein will block the lacI+pL promoter so that no cI is produced. Thus downstream (not included in this composite) the Pr promoter will be constitutively active, producing some zinc finger proteins, while Prm+ will have low or no activity.
STATE 2: when IPTG is present, it will remove the lacI protein from the lacI+pL promoter and cI is produced. Thus downstream (not included in this composite) the Pr promoter will be repressed by cI, while Prm+ will be activated, producing some other zinc finger proteins