EPD

BBa_J05500 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J05500
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ETH Zurich iGEM Team 2005
Date created: 2005-09-20 11:00:00
Date modified: 2015-08-31 04:08:15

Sensing Device A (cI)



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_J05500_sequence (Version 1)

Description

The input system for the iGEM2005 ETH "Counter" project: its purpose is to regulate cI production dependent on an external event. It works somehow like an inverter:


STATE 1: when IPTG is absent, the constitutively produced lacI protein will block the lacI+pL promoter so that no cI is produced. Thus downstream (not included in this composite) the Pr promoter will be constitutively active, producing some zinc finger proteins, while Prm+ will have low or no activity.


STATE 2: when IPTG is present, it will remove the lacI protein from the lacI+pL promoter and cI is produced. Thus downstream (not included in this composite) the Pr promoter will be repressed by cI, while Prm+ will be activated, producing some other zinc finger proteins

Notes

For more details see Sensing Device and Event Processing Device documentation.

Source

Registry 7.05

igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J05500/1