BBa_J100050

BBa_J100050 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_J100050

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Malcolm Campbell

Date created: 2012-01-10 12:00:00

Date modified: 2015-08-31 04:08:22

pBad+BD18+RFP_withoutATG

Details

• Types: DnaRegion
• Roles: engineered_region; Measurement
• Sequences: BBa_J100050_sequence (Version 1)

Description

This construct allows users to compare the effect of the pBad promtoer with two other constructs with similar design but different promoters. It contains the C-dog bicistronic piece J119024 plus RFP (E1010) but the original start codon has been deleted since BD18 provides a new start codon.

Notes

We had to delete the start codon from E1010 to make sure the final protein was the same as E1010.

Source

other parts in the registry

Components

Access	Instance	Definition
public	BBa_I13453	BBa_I13453
public	BBa_J119024	BBa_J119024
public	BBa_E1010	mRFP1

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I13453	1,130	BBa_I13453
BBa_J119024	139,226	BBa_J119024
BBa_E1010	233,913	mRFP1

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J100050/1

Member of these Collections

• iGEM Parts Registry