BBa_J100108

BBa_J100108 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J100108
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Erin McGuire
Date created: 2013-03-12 12:00:00
Date modified: 2015-08-31 04:08:22

Riboswitch and GFP +High production of eCDM8



    • Details

    Types
    DnaRegion

    Roles
    Generator

    engineered_region

    Sequences BBa_J100108_sequence (Version 1)

    Description

    High promotor and low RBS (c-Dog). E. coli optimized caffeine demethylase with the same amino acid sequence as yCDM8 from Michener and Smolke (2012). Theophylline sensitive riboswitch mediates the production of GFP.

    Notes

    J100079 was ligated using Spe1 and J100105 using Xba1, so there is no scar between the two parts and EcoR1

    Source

    eCDM8 was synthesized de novo.

    Access Instance Definition
    public
    public
    		BBa_J100079
    BBa_J100101
    		BBa_J100079
    BBa_J100101

    Sequence Annotation Location Component / Role(s)
    BBa_J100079
    BBa_J100101
    		1,879
    888,4182
    		BBa_J100079
    BBa_J100101

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J100108/1
     

    Attachments

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