Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Emanuel Nazareth
Date created: 2005-10-03 11:00:00
Date modified: 2015-08-31 04:08:25
Tunable Temperature Sensor v2 (using LacI ts + other parts from Registry 7.05)
| Types | DnaRegion |
| Roles | engineered_region Project |
| Sequences | BBa_J11021_sequence (Version 1) |
Description
Tuneable Temperature Sensor made with parts available in Registry 7.05 DNA-1. At higher temperatures, the lacI dimer degrades into lacI monomers. The LacI promoter is no longer inhibited, hence more RFP is produced. Also, cI 434 is produced resulting in Prm+ being repressed, thus lowering the levels of GFP. At lower temperatures, the lacI dimer is allowed to form, hence the LacI promoter is repressed. RFP levels decrease, and cI levels drop. This results in Prm+ no longer being repressed, hence GFP levels increase. Behaviour is tuneable by adjusting the levels of lacI ts via L-arabinose activating pBad. The net result is that the fluorescence (green to red) can be correlated to a temperature (at a given level of L-arabinose). Note IPTG induces R0010, offering a second degree of tuning.| Access | Instance | Definition |
| public public public public public public public public public public public public public public public public public public public public | BBa_I0500 BBa_B0034 BBa_J06501 BBa_B0010 BBa_B0012 BBa_R0010 BBa_B0031 BBa_C0056 BBa_B0010 BBa_B0012 BBa_I12006 BBa_B0032 BBa_E0040 BBa_B0010 BBa_B0012 BBa_R0010 BBa_B0031 BBa_E1010 BBa_B0010 BBa_B0012 | pBad/araC BBa_B0034 lacI ts BBa_B0010 BBa_B0012 LacI BBa_B0031 BBa_C0056 BBa_B0010 BBa_B0012 Prm + BBa_B0032 GFP BBa_B0010 BBa_B0012 LacI BBa_B0031 mRFP1 BBa_B0010 BBa_B0012 |