Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cody Barta
Date created: 2012-01-26 12:00:00
Date modified: 2015-08-31 04:08:26
pLacI-RBS-T7 RNAP + RFPrev-RBSrev-pT7rev
| Types | DnaRegion |
| Roles | engineered_region Measurement |
| Sequences | BBa_J119054_sequence (Version 1) |
Description
T7 RNA Polymerase expression driven by pLacI promoter. T7 RNAP gene has AscI and AsiSI sites for insertion of frameshift mutation oligos. T7 RNAP is able to drive expression of RFP from the pT7 promoter.Notes
Trascription of the reporter gene is leftward to avoid readthrough transcription of RFP from T7 RNAP gene.Source
Existing parts.| Access | Instance | Definition |
| public public | BBa_J119057 BBa_J119018 | BBa_J119057 BBa_J119018 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J119057 BBa_J119018 | 1,2827 2836,3590 | BBa_J119057 BBa_J119018 |