Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris French
Date created: 2007-07-12 11:00:00
Date modified: 2015-08-31 04:08:32
rbs+lacZ'
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_J15202_sequence (Version 1) |
Description
Escherichia coli lacZ' gene encoding the first 77 amino acids of LacZ (beta galactosidase), with a strong synthetic ribosome binding site. Complements the common lacZ-delta-M15 mutation found in many laboratory strains of E. coli, allowing blue-white selection. Note the presence of a SacI site at the 5' end, overlapping the XbaI site; this is preserved when this biobrick is added to any other biobrick.Notes
Note the presence of a SacI site at the 5' end, overlapping the XbaI site; this is preserved when this biobrick is added to any other biobrick. The lacZ' gene was truncated by alteration of codon 77 from TGC to TGA (note: not TAATAA as recommended, due to an error; however, the part works fine).Source
RBS synthetic; lacZ' from Escherichia coli BS, sequence derived from gi:146575.| Access | Instance | Definition |
| public public | BBa_J15001 BBa_J15103 | BBa_J15001 BBa_J15103 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J15001 BBa_J15103 | 1,10 17,247 | BBa_J15001 BBa_J15103 |