BBa_J18933

BBa_J18933 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J18933
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Raik Gruenberg
Date created: 2010-01-26 12:00:00
Date modified: 2015-08-31 04:08:36

FKBP~GLucFrag1



Types
DnaRegion

Roles
Translational_Unit

engineered_region

Sequences BBa_J18933_sequence (Version 1)

Description

P01 expression cassette
from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture:

  • T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop


Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts.

Expression



Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity.

Reference



  • Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices


Notes

  • Parts were assembled according to R.F.C. 25.
  • All parts were codon optimized for E. coli


Source

gene synthesis

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BBa_J18933/1