Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Raik Gruenberg
Date created: 2010-01-26 12:00:00
Date modified: 2015-08-31 04:08:36
FRB ~ GLucFrag2
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_J18941_sequence (Version 1) |
Description
P09 expression cassettefrom Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture:
- T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop
Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts.
Expression
Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity.
Reference
- Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
Notes
Constructs were assembled according to R.F.C. 25.All parts were codon optimized for E. coli.
Source
synthetic| Access | Instance | Definition |
| public public public public public public public public public public public public public | BBa_J18912 BBa_B0105 BBa_J18926 BBa_B0105 BBa_J18922 BBa_B0105 BBa_J18929 BBa_B0105 BBa_J18919 BBa_B0105 BBa_J18909 BBa_B0105 BBa_J18913 | T7start Scar 25 FRB Scar 25 5xGS Scar 25 GLucFrag2 Scar 25 preSCsite Scar 25 HisTag Scar 25 T7stop |