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BBa_J3108

BBa_J3108 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J3108

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Karmella Haynes

Date created: 2006-10-11 11:00:00

Date modified: 2015-08-31 04:08:45

AraC-PC, pLac-HinLVA

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Details

Types	DnaRegion
Roles	Generator engineered_region
Sequences	BBa_J3108_sequence (Version 1)

Description

Should constitutively express the AraC protein (pBad inhibitor). HinLVA is under the control of pLac. Therefore, HinLVA should be expressed in the presence of IPTG.

Notes

The double forward terminator is placed uptream of pLac-HinLVA to prevent forward read-through transcription from the pSB1AK3 vector.

Source

AraC-PC-TT - part BBa_S03591

pLac-HinLVA - part BBa_S03536

Components

Access	Instance	Definition
public public public public public public public public	BBa_I13458 BBa_B0010 BBa_B0012 BBa_R0010 BBa_B0030 BBa_J31001 BBa_B0010 BBa_B0012	BBa_I13458 BBa_B0010 BBa_B0012 LacI BBa_B0030 Hin LVA BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I13458 BBa_B0010 BBa_B0012 BBa_R0010 BBa_B0030 BBa_J31001 BBa_B0010 BBa_B0012	1,1114 1123,1202 1211,1251 1260,1459 1468,1482 1489,2100 2109,2188 2197,2237	BBa_I13458 BBa_B0010 BBa_B0012 LacI BBa_B0030 Hin LVA BBa_B0010 BBa_B0012

Other Properties

igem#experience	Works
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_J3108/1