Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Perry Tsai
Date created: 2006-10-28 11:00:00
Date modified: 2015-08-31 04:08:47
Lipoprotein signal peptide + Outer membrane protein A, aa 46-159
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_J36839_sequence (Version 1) |
Description
This part is an assembly of the lipoprotein 20aa signal peptide and its 1st nine amino acids, with amino acids 46-159 of outer membrane protein A. When fused with a protein downstream, this surface display vehicle should express a protein on the outer surface of E. coli.NOTE ABOUT THE SEQUENCE: The mixed site between parts is 'only' six base pairs, ACTAGA. There is no spacer T or G nucleotide. These spacer nucleotides have been placed in the results for "get selected sequence" as an automatic composite-parts addition for the BioBricks mixed site between assembled parts.
Notes
There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains.Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts.
Source
Assembly of BBa_J36835 and BBa_J36837| Access | Instance | Definition |
| public public | BBa_J36835 BBa_J36837 | BBa_J36835 BBa_J36837 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J36835 BBa_J36837 | 1,87 96,437 | BBa_J36835 BBa_J36837 |