Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Perry Tsai
Date created: 2006-10-30 12:00:00
Date modified: 2015-08-31 04:08:47
Lac-inducible generator of Lpp-OmpA(46-66)-Streptavidin wild-type + His6tag
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_J36848_sequence (Version 1) |
Description
This device contains a lac promoter and strong ribosome binding site for lac-inducible expression of the fusion protein of Lpp signal peptide, OmpA aa46-66, and streptavidin wild-type + His6 tag. This expression should display streptavidin on the cell surface of E. coli.NOTE ABOUT THE SEQUENCE: The mixed site between parts is 'only' six base pairs, ACTAGA. There is no spacer T or G nucleotide. These spacer nucleotides have been placed in the results for "get selected sequence" as an automatic composite-parts addition for the BioBricks mixed site between assembled parts. However, this does not apply between the R0010 and B0031 in the J04500 composite because that was assembled in a standard fashion.
Notes
There is no spacer nucleotide between the end of the device and the SpeI restriction site.Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts. This does not apply between R0010 and B0031.
Source
Assembly of BBa_J04500 and BBa_J36844| Access | Instance | Definition |
| public public public public public | BBa_R0010 BBa_B0034 BBa_J36835 BBa_J36836 BBa_J36841 | LacI BBa_B0034 BBa_J36835 BBa_J36836 BBa_J36841 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 BBa_B0034 BBa_J36835 BBa_J36836 BBa_J36841 | 1,200 209,220 227,313 322,384 393,797 | LacI BBa_B0034 BBa_J36835 BBa_J36836 BBa_J36841 |