| Types || |
| Roles |
| Sequences || BBa_J36848_sequence (Version 1)|
DescriptionThis device contains a lac promoter and strong ribosome binding site for lac-inducible expression of the fusion protein of Lpp signal peptide, OmpA aa46-66, and streptavidin wild-type + His6 tag. This expression should display streptavidin on the cell surface of E. coli.
NOTE ABOUT THE SEQUENCE: The mixed site between parts is 'only' six base pairs, ACTAGA. There is no spacer T or G nucleotide. These spacer nucleotides have been placed in the results for "get selected sequence" as an automatic composite-parts addition for the BioBricks mixed site between assembled parts. However, this does not apply between the R0010 and B0031 in the J04500 composite because that was assembled in a standard fashion.
NotesThere is no spacer nucleotide between the end of the device and the SpeI restriction site.
Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts. This does not apply between R0010 and B0031.
SourceAssembly of BBa_J04500 and BBa_J36844