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exp. GFP

BBa_J45996 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J45996

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Stephen Payne

Date created: 2006-10-30 12:00:00

Date modified: 2015-08-31 04:08:50

Exponential phase dependent GFP generator

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Details

Types	DnaRegion
Roles	engineered_region Generator
Sequences	BBa_J45996_sequence (Version 1)

Description

This composite part consists of J45992 (a stationary phase promoter), Q04401 (an inverter), and E0840 (a green fluorescent protein generator). This device expresses GFP in the exponential phase of cell growth.

Notes

This device was constructed to test whether the J45180 device would work.

Source

J45992 was PCRed out of the E. coli genome, while Q04401 and E0840 were developed by others in the Registry.

Components

Access	Instance	Definition
public public public public public public	BBa_J45992 BBa_Q04401 BBa_B0030 BBa_E0040 BBa_B0010 BBa_B0012	p(osmY) tetR inver BBa_B0030 GFP BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J45992 BBa_Q04401 BBa_B0030 BBa_E0040 BBa_B0010 BBa_B0012	1,199 208,1109 1118,1132 1139,1858 1867,1946 1955,1995	p(osmY) tetR inver BBa_B0030 GFP BBa_B0010 BBa_B0012

Other Properties

igem#experience	Works
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_J45996/1