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BBa_J5517

BBa_J5517 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J5517

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Charles Yoon

Date created: 2006-10-28 11:00:00

Date modified: 2015-08-31 03:22:59

Arabinose -> LacI ts -| GFP

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Details

Types	DnaRegion
Roles	Measurement engineered_region
Sequences	BBa_J5517_sequence (Version 1)

Description

When induced by arabinose, temperature sensitive LacI (LacI ts) will be produced. LacI has both a dimer and tetramer form. These forms are in equilibrium and this equilibrium can be shifted with heat. As more LacI ts tetramer is formed, pLac will be repressed, thus producing less green fluorescent protein (GFP).

Notes

The fluorescence of GFP relative to the auto-fluorescence of untransformed cells.

Source

iGEM 2006 Registry

Components

Access	Instance	Definition
public public public public public public public public public public	BBa_I0500 BBa_B0034 BBa_J06501 BBa_B0010 BBa_B0012 BBa_R0011 BBa_B0032 BBa_E0040 BBa_B0010 BBa_B0012	pBad/araC BBa_B0034 lacI ts BBa_B0010 BBa_B0012 lacI+pL BBa_B0032 GFP BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I0500 BBa_B0034 BBa_J06501 BBa_B0010 BBa_B0012 BBa_R0011 BBa_B0032 BBa_E0040 BBa_B0010 BBa_B0012	1,1210 1219,1230 1237,2389 2398,2477 2486,2526 2535,2588 2598,2610 2617,3336 3345,3424 3433,3473	pBad/araC BBa_B0034 lacI ts BBa_B0010 BBa_B0012 lacI+pL BBa_B0032 GFP BBa_B0010 BBa_B0012

Other Properties

igem#experience	Works
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_J5517/1