Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lorenzo Pasotti, Paolo Magni
Date created: 2008-10-18 11:00:00
Date modified: 2015-05-08 01:08:34
Mux SEL
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K081002_sequence (Version 1) |
Description
This composite part is the union of:*a PoPS in -> cI, lasR out device and
*a Plambda-regulated luxR protein generator.
It is a module of the following genetic multiplexer (mux):
{|align="center"
|[[Image:Pv_mux_final.png|500px]]
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|[[Image:pv_muxregistry_interactions.png|500px]]
|}
where:
*PA, PS and PB are three promoters that can be ACTIVATED by the three generic elements A, S and B respectively;
*GOI is the gene of interest that is the output of the system.
In particular, K081003 is the Selector.
People who want to use the genetic mux have to assemble:
*K081000 under the desired regulation for Channel 0 (for example the generic promoter PA);
*K081001 under the desired regulation for Channel 1 (for example the generic promoter PB);
*K081002 under the desired regulation for Selector (for example the generic promoter PS);
*the desired protein generator downstream of K081000 and K081001 (for example the generic RBS-GOI-T generator);
Notes
We used BioBrick Standard Assembly.Source
RBS: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.cI, Plambda: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross.
lasR: Alvin Carter Powers (Fighting Darwins).
T: Haiyao Huang.
luxR: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr.