BBa_K090402

BBa_K090402 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K090402

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Daniel Goodman

Date created: 2008-10-29 12:00:00

Date modified: 2015-06-09 09:16:11

B. subtilis Episomal Vector with Constitutive GFP

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K090402_sequence (Version 1)

Description

This episomal vector has within its BioBrick region a constitutively expressed GFP gene. It uses the pUpp strong constitutive promoter and a consensus Bacillus RBS sequence.

Notes

To build this part, we used 4 different linear DNA pieces assembled with 15 base pair homologous regions PCRed to the ends. These 4 different pieces were then assembled via the InFusion assembly method by Clontech.

Source

See data for BBa_K90401. This part is the same, but with the pUpp promoter, a gram-positive RBS, and a GFP with terminator.

Components

Access	Instance	Definition
public	BBa_K090401	pBSEP1
public	BBa_K090504	BBa_K090504
public	BBa_K090505	BBa_K090505
public	BBa_E0040	GFP
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K090401	1,5208	pBSEP1
BBa_K090504	5217,5455	BBa_K090504
BBa_K090505	5464,5474	BBa_K090505
BBa_E0040	5481,6200	GFP
BBa_B0010	6209,6288	BBa_B0010
BBa_B0012	6297,6337	BBa_B0012

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K090402/1

Member of these Collections

• //chassis/prokaryote/bsubtilis
• //plasmid
• iGEM Parts Registry