PN+E0240

BBa_K100004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K100004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Garrett Tobin

Date created: 2008-08-17 11:00:00

Date modified: 2015-05-08 01:08:41

Natural Xylose Regulated Bi-Directional Operator + GFP

Details

• Types: DnaRegion
• Roles: engineered_region; Measurement
• Sequences: BBa_K100004_sequence (Version 1)

Description

This part is the Natural Xylose Regulated Bi-Directional Operator (BBa_K100000) followed by an RBS GFP Termonator (BBa_E0240). This composite part measures activation of the promoter, used to determining if xylose-glucose diauxie is eliminated.

Notes

GFP was use so that fluorescent strength could be used to measure induction.

Source

composite - See BBa_E0240 and BBa_K100000

Components

Access	Instance	Definition
public	BBa_K100000	PN
public	BBa_B0032	BBa_B0032
public	BBa_E0040	GFP
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K100000	1,303	PN
BBa_B0032	312,324	BBa_B0032
BBa_E0040	331,1050	GFP
BBa_B0010	1059,1138	BBa_B0010
BBa_B0012	1147,1187	BBa_B0012

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K100004/1

Member of these Collections

• iGEM Parts Registry