Blue gR4mR

BBa_K1026010 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1026010
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hongyi WU
Date created: 2013-09-26 11:00:00
Date modified: 2015-05-08 01:08:46

A gRNA targeting mRFP, negatively regulated by Blue Light Sensor



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1026010_sequence (Version 1)

Description

This gRNA targeting mRFP is a no-BioBrick-scars assembly of the red light regulated promoter BBa_K592006 (PFixK2), DNA sequence of a gRNA that targets mRFP reporter and a reliable terminator BBa_B0015. This gRNA sequence comes from the following literature: QI, LEI S., LARSON, MATTHEW H., GILBERT, LUKE A., DOUDNA, JENNIFER A., WEISSMAN, JONATHAN S., ARKIN, ADAM P. & LIM, WENDELL A. 2013. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell, 152, 1173-1183.

Notes

gRNA shall be exactly transcribed out. Any extra nucleotides would potentially jeopardize the normal function of the gRNA. Therefore, BioBrick scars are not allowed here. Instead of cut-and-ligation, overlap PCR is preferred for the assembly task (changing promoter). In addition, a reliable terminator is applied here to ensure the termination of gRNA.

Source

The gRNA sequence is de novo synthesized.

igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1026010/1