Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jama Hagi-Yusuf
Date created: 2013-09-12 11:00:00
Date modified: 2015-05-08 01:08:50
BXB1 integrase under lock and key control with lacI expression cassette
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1039023_sequence (Version 1) |
Description
Bxb1 integrase (BBa_K1039003) and lacI repressor (BBa_Q04121) are under the constitutive strong promoter BBa_J23119. Key (BBa_I714036) is under the lac inducible promoter BBa_R0011. BBa_B0015, the transcriptional terminator, is after every Bxb1 integrase and lacI repressor cassette.Bxb1 integrase with ribosome binding sites associated with the cis-repressive RNA ???lock??? (BBa_J01010), so translation is inhibited. The trans-activating RNA ???key??? can displace the lock and allows translation.
Notes
For Bxb1 integrase, the following three silent mutations were made to remove forbidden restriction sites:base 273 G->A
base 1269 A->T
base 1440 G->A
Source
Some of these parts were synthesized by BioBasic Inc and some were obtained from the iGEM 2013 distribution kit.| Access | Instance | Definition |
| public public public public public public public public | BBa_J23119 BBa_Q04121 BBa_J01010 BBa_K1039003 BBa_B0015 BBa_R0011 BBa_I714036 BBa_B0015 | BBa_J23119 lacI Lock 1 Bxb1-int BBa_B0015 lacI+pL BBa_I714036 BBa_B0015 |