Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eri Takagi
Date created: 2013-10-10 11:00:00
Date modified: 2015-05-08 01:08:56
Pconst.(H)-RBS-FA-linker-MS2-RBS-FB-linker-PP7-double terminator
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1053106_sequence (Version 1) |
Description
Split GFP N-terminal domain (FA) is bound to MS2 through linker. Split GFP is consisted of two half GFP; FA (N-terminal domain) and FB (C-terminal domain) between amino acid residue 158 and 159. Either FA or FB is not able to emit fluorescence. When FA and FB come closer, the fragments associate and restore fluorescence. Also, MS2 is the single strand RNA phage coat protein which is bound specifically by MS2 aptamer.Notes
The synthesized three-part sequence for FA-linker-MS2 was ligated by overlap PCR.Source
We used the synthesized sequences of split GFP fused with MS2 and PP7 (FA-linker-MS2, FB-linker-PP7) respectively, reference of BioBrick part BBa_K738004 (FA-linker-MS2) and BBa_K738005 (FB-linker-PP7) (ZJU-China 2012).| Access | Instance | Definition |
| public public public public public public public | BBa_J23100 BBa_B0034 BBa_K1053103 BBa_B0034 BBa_K1053104 BBa_B0010 BBa_B0012 | BBa_J23100 BBa_B0034 BBa_K1053103 BBa_B0034 BBa_K1053104 BBa_B0010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_B0034 BBa_K1053103 BBa_B0034 BBa_K1053104 BBa_B0010 BBa_B0012 | 1,35 44,55 64,1346 1355,1366 1373,2026 2035,2114 2123,2163 | BBa_J23100 BBa_B0034 BBa_K1053103 BBa_B0034 BBa_K1053104 BBa_B0010 BBa_B0012 |