Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ingmar Claes
Date created: 2013-09-23 11:00:00
Date modified: 2015-05-08 01:08:58
Methyl Salicylate Production under TetR regulation
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1060012_sequence (Version 1) |
Description
KU_Leuven 2013 requested the BBa_J45700 brick from MIT 2006 and recloned into the pSB1C3 standard plasmid backbone (BBa_K1060003). In this part we removed the lac promoter in front of the pchBA gene to get a constitutive expression of all genes needed for methyl salicylate production. We did this by amplifying the For the assembly of this part we refer to the original MIT brick BBa_J45700 => "We assembled a complete wintergreen odor biosynthetic system (BBa_J45700) by combining the salicylic acid (BBa_J45320) and wintergreen odor generators (BBa_J45120). BBa_J45700 catalyzes the conversion of the cellular metabolite chorismate to methyl salicylate or wintergreen odor."Notes
no EcoRI, XbaI, NotI, SpeI or PstI sites presentSource
BBa_K1060003, BBa_C0040| Access | Instance | Definition |
| public public public public public public public public | BBa_R0040 BBa_B0030 BBa_J45004 BBa_K823017 BBa_R0040 BBa_B0032 BBa_J45017 BBa_B0015 | p(tetR) BBa_B0030 BSMT1 BBa_K823017 p(tetR) BBa_B0032 pchBA BBa_B0015 |