Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gabriele Girelli
Date created: 2013-08-13 11:00:00
Date modified: 2015-05-08 01:08:59
lac_promoter + RBS + SAM synthetase
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1065105_sequence (Version 1) |
Description
SAM synthetase enzyme catalyzes the reaction of synthesis of S-AdoMet. RBS is 'AAGGAGG'.Notes
The gene sequence was extracted using the following primers:>Primer Forward: prefix + RBS + spacer + ATG...
GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT
>Primer Reverse: sequence_TAATAA_suffix
GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT
Source
SAM synthetase gene sequence extracted by PCR from E. coli MG1655 strain genome.| Access | Instance | Definition |
| public public | BBa_R0010 BBa_K1065104 | LacI BBa_K1065104 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 BBa_K1065104 | 1,200 209,1377 | LacI BBa_K1065104 |