Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Viola Valentini
Date created: 2013-08-31 11:00:00
Date modified: 2015-05-08 01:08:59
Efe+Bba_B0015 in BBa_K823024 (pXyl)
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1065204_sequence (Version 1) |
Description
2-oxoglutarate oxygenase/decarboxylase is an Ethylene Forming Enzyme (EFE) that catalyze Ethylene biosynthesis from 2-oxoglutarate. This part is in the pXyl vector that has been sent to the Igem UNITN 2013 team from the LMU Munich 2012 team. This part was cloned by the iGEM Trento 2013 team for the creation of an aerobically engineered pathway for the control of fruit ripening. Further information about this part and its characterization can be found in the iGEM Trento 2013 wiki.Notes
Design Notes
The coding sequence was taken from Pseudomonas syringae pv. phaseolicola PK2. We firstly optimized the codons for both B. subtilis and E. coli and then synthesized the gene with an RBS sequence upstream of the start codon. We decided to include in the CDS two restriction sites (NgoMIV and AgeI) in order to allow people to use the Freiburg assembly.
GAATTCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGCACC...
...TCGACCGGTTAATACTAGTAGCGGCCGCTGCAG
Source
This enzyme was firstly purified from Pseudomonas Siringae pv. phaseolicola PK2, a 2-oxoglutarate-dependent ethylene producing bacterium [1]. The CDS has been optimized for both E.coli and B.subtilis. We decided to include an RBS sequence too.| Access | Instance | Definition |
| public public | BBa_K1065002 BBa_K823024 | EFE + T pSBBs4S |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1065002 BBa_K823024 | 1,1215 1224,6017 | EFE + T pSBBs4S |