Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Junqi kuang, Yaqing du and Junjie zhang
Date created: 2013-09-07 11:00:00
Date modified: 2015-05-08 01:09:01
Pgpa1+Chimeric Gpa1(Gα
subunit)+Rfp+ADH1
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1072012_sequence (Version 1) |
Description
In yeast, as in mammalian cells, the pheromone receptors are coupled to a heterotrimeric G protein. This is composed of Gpa1p (Gα), Ste4p (Gβ) and Ste18p (Gγ) subunits. In order to coupling heterologous GPCRs in yeast, ???Transplants??? have the five C-terminal residues,originating from a C. elegans, G- protein, Odr3, replaced with the corresponding mammalian Gα residues.
Figure 1. Comperison between endogenous GPCR pathway in yeast and motified Odr10 pathway.
a repetor gene, Rfp, and ADH1 terminator have been added to de
Notes
-Source
-| Access | Instance | Definition |
| public public public | BBa_K1072011 BBa_E1010 BBa_K801012 | BBa_K1072011 mRFP1 BBa_K801012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1072011 BBa_E1010 BBa_K801012 | 1,2419 2426,3131 3140,3488 | BBa_K1072011 mRFP1 BBa_K801012 |