Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM Team Braunschweig 2013
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:09:02
Autoinducer synthase LuxI + LVA with combined amilGFP chromoprotein expression cassette
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1073031_sequence (Version 1) |
Description
This part is the basis for the expression of the Lux auto inducer synthetase, which synthesizes LuxI [N-(3-oxohexanoyl)-homoserine lactone]. LuxI binds to the activator LuxR. Together they activate the Lux promoter. A constitutive expression of amil GFP is added to the construct in order to varify the expression of LuxI synthetase. The construct has no promoter in front of the LuxI synthetase gene. Therefore the user can choose the promoter himself (e.g. constitutive, induced, repressed, etc.)Notes
no considerations.Source
The biobricks were taken from the 2013 distribution kit and the iGEM Team Uppsala.| Access | Instance | Definition |
| public public public | BBa_K1073017 BBa_K1073024 BBa_B0015 | BBa_K1073017 BBa_K1073024 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1073017 BBa_K1073024 BBa_B0015 | 1,799 808,1568 1577,1705 | BBa_K1073017 BBa_K1073024 BBa_B0015 |