Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM Team Braunschweig 2013
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:09:02
Inducible ampR expression combined with LasR, RhlI and aeBlue expression cassettes
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1073034_sequence (Version 1) |
Description
This construct is the basis for a Quorum sensing system for two bacteria. The selection marker (amp-resistance) is induced by LasI(inducer)/LasR(activator) complex. The LasR is encoded on the construct itself, whereas the LasI is taken up from the media. Furthermore the RhlI synthetase is expressed to synthesize the autoinducer RhlI for the corresponding system in another cell (BBa_K1073035).By the activation of the amp-resistance the gene of interest (in this case the chromoprotein aeblue) is expressed. Together with BBa_K1073035 the construct forms a dependency between two cells (one with BBa_K10733034, the other with BBa_K1073035).
Notes
no considerations.Source
The parts for this construct were derived from the 2013 distribution kit and the iGEM Team Uppsala.| Access | Instance | Definition |
| public public public | BBa_B0015 BBa_K1073030 BBa_K1073033 | BBa_B0015 BBa_K1073030 BBa_K1073033 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0015 BBa_K1073030 BBa_K1073033 | 1,129 138,2142 2151,3879 | BBa_B0015 BBa_K1073030 BBa_K1073033 |