Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Patrick Rosendahl Andreassen
Date created: 2013-09-18 11:00:00
Date modified: 2015-05-08 01:09:06
B. subtilis dxs-GFP protein fusion (lac promoter with lac inhibitor: IPTG inducible)
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1088026_sequence (Version 1) |
Description
This part consist of the dxs gene derived from B. subtilis fused to GFP at translational level with a 10 AA linker between the proteins. The reporter fusion is under the control of the lac promoter and has a strong RBS.To repress expression from the lac promoter, the lacI under a constitutive promoter, with a strong RBS and a efficient terminator is placed counter-clockwise to the reporter fusion. The repression can be relieved with addition of IPTG, which binds and inhibits the function of LacI.
The levels of expression was meassured in E. coli K-12 MG1655 using fluorescence activated cell sorting, and proved that their was no expression when grown without IPTG and that there was expression after addition of IPTG. See experience for more details.
This Brick was build to test induction time and IPTG concentration for induction of a similar device which lacks the linker and GFP (BBa_K1088027)
Notes
A linker was placed between the two genes to decrease the risk of misfolding and loss of function.Source
The components specify the source| Access | Instance | Definition |
| public public | BBa_K1088019 BBa_K1088008 | BBa_K1088019 BBa_K1088008 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1088019 BBa_K1088008 | 1,1173 1182,4049 | BBa_K1088019 BBa_K1088008 |