Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jake Awtry
Date created: 2013-09-11 11:00:00
Date modified: 2015-05-08 01:09:12
Level 1 MoClo Device that expresses tetR under pBAD
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1114516_sequence (Version 1) |
Description
pI134B2C40_AE
This is a Level 1 MoClo device that expresses YFP under the repressible promoter lacI. It contains the Level 0 parts J23104_AB, BCD2_BC, C0040_CD, and B0015_DE within the DVL1_AE backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.
Notes
pI134B2C40_AE
This is a Level 1 MoClo device that expresses YFP under the repressible promoter lacI. It contains the Level 0 parts J23104_AB, BCD2_BC, C0040_CD, and B0015_DE within the DVL1_AE backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.
Source
Construct of MoClo Level 0 parts derived from iGEM Distribution Kits| Access | Instance | Definition |
| public public public public | BBa_K1114005 BBa_K1114107 BBa_K1114202 BBa_K1114300 | BBa_K1114005 BBa_K1114107 BBa_K1114202 BBa_K1114300 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1114005 BBa_K1114107 BBa_K1114202 BBa_K1114300 | 1,43 52,143 152,816 825,961 | BBa_K1114005 BBa_K1114107 BBa_K1114202 BBa_K1114300 |