View

LasR-mCher

BBa_K1157014 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1157014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lin Ang Chieh

Date created: 2013-09-14 11:00:00

Date modified: 2015-05-08 01:09:30

pLas-LasR-GFPmut3-Pc-LasR

Back

Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_K1157014_sequence (Version 1)

Description

This biosensor is built to sense C-12 AHL which originates from Pseudomonas aeruginosa. It uses GFP as a reporter, and constitutively express Rhl receptor. When binding with AHL molecules, Rhl receptor will regulate promoter sequence(pRhl) and express GFP which can be detected.

Notes

These parts are built by igem official biobricks, so there is no big considerations of sequence design.

Source

This is built by the original biobricks provided by igem official

Components

Access	Instance	Definition
public public public public public public public public public public	BBa_R0079 BBa_B0030 BBa_C0179 BBa_B0030 BBa_E0040 BBa_B0015 BBa_J23100 BBa_B0030 BBa_C0179 BBa_B0015	LasR+PAI BBa_B0030 lasR BBa_B0030 GFP BBa_B0015 BBa_J23100 BBa_B0030 lasR BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0079 BBa_B0030 BBa_C0179 BBa_B0030 BBa_E0040 BBa_B0015 BBa_J23100 BBa_B0030 BBa_C0179 BBa_B0015	1,157 166,180 187,909 918,932 939,1658 1667,1795 1804,1838 1847,1861 1868,2590 2599,2727	LasR+PAI BBa_B0030 lasR BBa_B0030 GFP BBa_B0015 BBa_J23100 BBa_B0030 lasR BBa_B0015

Other Properties

igem#experience	None
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K1157014/1