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Las sensor

BBa_K1157016 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1157016

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lin Ang Chieh

Date created: 2013-09-14 11:00:00

Date modified: 2015-05-08 01:09:30

Pc-LasR-pLas-BFP

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_K1157016_sequence (Version 1)

Description

In this biosensor, we use a constitutive promoter to express quorum sensing receptor LasR. When 12-C AHL molecule binds with LasR, it is suppose to regulate the downstream reporter BFP, which is able to detect.

Notes

There are no detailed design sequences in this construct.

Source

This construct is built by parts given by iGEM official.

Components

Access	Instance	Definition
public public public public public public public public	BBa_J23100 BBa_B0030 BBa_C0179 BBa_B0015 BBa_R0079 BBa_B0030 BBa_K592100 BBa_B0015	BBa_J23100 BBa_B0030 lasR BBa_B0015 LasR+PAI BBa_B0030 BFP BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23100 BBa_B0030 BBa_C0179 BBa_B0015 BBa_R0079 BBa_B0030 BBa_K592100 BBa_B0015	1,35 44,58 65,787 796,924 933,1089 1098,1112 1119,1823 1832,1960	BBa_J23100 BBa_B0030 lasR BBa_B0015 LasR+PAI BBa_B0030 BFP BBa_B0015

Other Properties

igem#experience	None
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K1157016/1