pPQS-GFP

BBa_K1157017 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1157017

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lin Ang Chieh

Date created: 2013-09-14 11:00:00

Date modified: 2015-05-08 01:09:30

pPQS-GFPmut3

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1157017_sequence (Version 1)

Description

This is a sub-part of the quorum sensing molecule 2-heptyl-3-hydroxy-4-quinolone. It is composed by a promoter sequence of PQS and a reporter GFPmut3.

Notes

There are no restriction sites in the inner part of the DNA so there are no specific design to the sequence.

Source

pPQS is originally made through PCR of whole genome Pseudomonas aeruginosa ATCC 27853. The reporter is constructed by igem biobricks from iGEM official.

Components

Access	Instance	Definition
public	BBa_K1157000	pPQS
public	BBa_B0030	BBa_B0030
public	BBa_E0040	GFP
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1157000	1,341	pPQS
BBa_B0030	350,364	BBa_B0030
BBa_E0040	371,1090	GFP
BBa_B0015	1099,1227	BBa_B0015

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1157017/1

Member of these Collections

• iGEM Parts Registry