Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lin Ang Chieh
Date created: 2013-09-14 11:00:00
Date modified: 2015-05-08 01:09:30
Pc-PQSR
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1157019_sequence (Version 1) |
Description
This is a sub-part of quorum sensing molecule biosensor 2-heptyl-3-hydroxy-4(1H)-quinolone sensed by PQS receptor. The constitutive promoter expresses PQS receptor, and in the final construct, if the PQS receptor(PQSR) binds with 2-heptyl-3-hydroxy-4(1H)-quinolone, it will be able to regulate its promoter sequence resulting in expression of reporter mCherry.Notes
There are no restriction sites inside the sequence.Source
PQSR is a pcr product of Pseudomonas aeruginosa ATCC 27853. The rest is provided by iGEM official biobrick.| Access | Instance | Definition |
| public public public public | BBa_J23119 BBa_B0030 BBa_K1157001 BBa_B0015 | BBa_J23119 BBa_B0030 PQSR BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23119 BBa_B0030 BBa_K1157001 BBa_B0015 | 1,35 44,58 65,1063 1072,1200 | BBa_J23119 BBa_B0030 PQSR BBa_B0015 |