BBa_K1157020

BBa_K1157020 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1157020
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lin Ang Chieh
Date created: 2013-09-14 11:00:00
Date modified: 2015-05-08 01:09:30

Pc-RhlR-pRhl-RhlR-GFPmut3



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1157020_sequence (Version 1)

Description

This biosensor is built to sense C-4 AHL molecules. The constitutive promoter continues to express Rhl receptor(RhlR), and as the Rhl receptor binds with C-4 AHL, it binds with promoter sequence of Rhl and produces more Rhl receptor and reporter GFP which can be detected. The adding of a Rhl receptor before GFP is to induce a positive circuit in order to magnify the signal.

Notes

The sequences are confirmed and there is no restriction enzyme sites in the sequence.

Source

It is built by iGEM official biobrick

igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1157020/1