Types | DnaRegion
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Roles | engineered_region
Composite
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Sequences | BBa_K1157020_sequence (Version 1)
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Description
This biosensor is built to sense C-4 AHL molecules. The constitutive promoter continues to express Rhl receptor(RhlR), and as the Rhl receptor binds with C-4 AHL, it binds with promoter sequence of Rhl and produces more Rhl receptor and reporter GFP which can be detected. The adding of a Rhl receptor before GFP is to induce a positive circuit in order to magnify the signal.
Notes
The sequences are confirmed and there is no restriction enzyme sites in the sequence.
Source
It is built by iGEM official biobrick